PrimePCR™ SYBR® Green Assay: PLCXD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLCXD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLCXD1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011647
Assay Design:   Intron-spanning
Chromosome Location:   X:200905-205455question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000399012 ENST00000430923 ENST00000445062 ENST00000381657 ENST00000429181 ENST00000443019 ENST00000415337 ENST00000447472 ENST00000448477 ENST00000381663

Gene Information

This gene is the most terminal protein-coding gene in the pseudoautosomal (PAR) region on chromosomes X and Y. Alternate splicing results in multiple transcript variants. [provided by RefSeq Mar 2010]

Gene Symbol:   PLCXD1
Gene Name:   phosphatidylinositol-specific phospholipase C, X domain containing 1
Aliases:   FLJ11323
RefSeq:   NC_000023.10 NC_000024.9 NG_016868.1 NT_167192.1 NT_167200.1
Ensembl:   ENSG00000182378
Entrez:   55344
Chromosome Mapping:   Xp22.33; Yp11.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 33.340000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download