This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028115
PrimePCR™ PreAmp for SYBR® Green Assay: MTHFD1, Human
PrimePCR™ Template for SYBR® Green Assay: MTHFD1, Human
This gene encodes a protein that possesses three distinct enzymatic activities 510-methylenetetrahydrofolate dehydrogenase 510-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate which are substrates for methionine thymidylate and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.