PrimePCR™ SYBR® Green Assay: VRK1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: VRK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VRK1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011636
Assay Design:   Intron-spanning
Chromosome Location:   14:97321619-97322525question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000216639 ENST00000557222

Gene Information

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells such as those in testis thymus fetal liver and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and in vitro to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene therefore may regulate cell proliferation. This protein also phosphorylates histone casein and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq Jul 2008]

Gene Symbol:   VRK1
Gene Name:   vaccinia related kinase 1
Aliases:   MGC117401, MGC138280, MGC142070, PCH1
RefSeq:   NC_000014.8 NG_016293.1 NT_026437.12
Ensembl:   ENSG00000100749
Entrez:   7443
Chromosome Mapping:   14q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download