This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CDH16, Human
PrimePCR™ Template for SYBR® Green Assay: CDH16, Human
This gene is a member of the cadherin superfamily genes encoding calcium-dependent membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1 the gene localizes with superfamily members CDH1 CDH3 CDH5 CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney where the protein functions as the principal mediator of homotypic cellular recognition playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Mar 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.