PrimePCR™ SYBR® Green Assay: CDH16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011589
Assay Design:   Intron-spanning
Chromosome Location:   16:66947448-66948169question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000544875 ENST00000394055 ENST00000299752

Gene Information

This gene is a member of the cadherin superfamily genes encoding calcium-dependent membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1 the gene localizes with superfamily members CDH1 CDH3 CDH5 CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney where the protein functions as the principal mediator of homotypic cellular recognition playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Mar 2011]

Gene Symbol:   CDH16
Gene Name:   cadherin 16, KSP-cadherin
Aliases:   Not Available
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000166589
Entrez:   1014
UniGene:   Hs.513660
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.260000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download