This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028087
PrimePCR™ PreAmp for SYBR® Green Assay: SCD5, Human
PrimePCR™ Template for SYBR® Green Assay: SCD5, Human
Stearoyl-CoA desaturase (SCD; EC 18.104.22.168) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms Scd1 through Scd4 have been identified in mouse. In contrast only 2 SCD isoforms SCD1 (MIM 604031) and SCD5 have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms as well as with rat Scd1 and Scd2. In contrast SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al. 2005 [PubMed 15907797]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.