PrimePCR™ SYBR® Green Assay: KHSRP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028079

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KHSRP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KHSRP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011555
Assay Design:   Intron-spanning
Chromosome Location:   19:6416998-6417790question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000201886 ENST00000398148

Gene Information

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes including transcription alternative pre-mRNA splicing and mRNA localization (Min et al. 1997 [PubMed 9136930]; Gherzi et al. 2004 [PubMed 15175153]).[supplied by OMIM Apr 2010]

Gene Symbol:   KHSRP
Gene Name:   KH-type splicing regulatory protein
Aliases:   FBP2, FUBP2, KSRP, MGC99676
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000088247
Entrez:   8570
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 32.900000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download