This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028079
PrimePCR™ PreAmp for SYBR® Green Assay: KHSRP, Human
PrimePCR™ Template for SYBR® Green Assay: KHSRP, Human
The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes including transcription alternative pre-mRNA splicing and mRNA localization (Min et al. 1997 [PubMed 9136930]; Gherzi et al. 2004 [PubMed 15175153]).[supplied by OMIM Apr 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.