PrimePCR™ SYBR® Green Assay: FBXL16, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028078

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011554
Assay Design:   Intron-spanning
Chromosome Location:   16:745882-746856question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000324361 ENST00000397621

Gene Information

Members of the F-box protein family such as FBXL16 are characterized by an approximately 40-amino acid F-box motif. SCF complexes formed by SKP1 (MIM 601434) cullin (see CUL1; MIM 603134) and F-box proteins act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box and they interact with ubiquitination targets through other protein interaction domains (Jin et al. 2004 [PubMed 15520277]).[supplied by OMIM Mar 2008]

Gene Symbol:   FBXL16
Gene Name:   F-box and leucine-rich repeat protein 16
Aliases:   C16orf22, FLJ33735, Fbl16, MGC33974, c380A1.1
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000127585
Entrez:   146330
UniGene:   Hs.513244
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.790000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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