This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028071
PrimePCR™ PreAmp for SYBR® Green Assay: DIRAS3, Human
PrimePCR™ Template for SYBR® Green Assay: DIRAS3, Human
This gene is a member of the ras superfamily and is expressed in normal ovarian and breast epithelial cells but not in ovarian and breast cancers. It is an imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. Thus this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq Oct 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.