This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0028064
PrimePCR™ PreAmp for SYBR® Green Assay: RFX1, Human
PrimePCR™ Template for SYBR® Green Assay: RFX1, Human
This gene is a member of the regulatory factor X gene family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2 X3 X4 and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2 X3 and X5 but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.