PrimePCR™ SYBR® Green Assay: LAPTM4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011473
Assay Design:   Intron-spanning
Chromosome Location:   2:20234765-20237196question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000175091

Gene Information

This gene encodes a protein that has four predicted transmembrane domains. The function of this gene has not yet been determined; however studies in the mouse homolog suggest a role in the transport of small molecules across endosomal and lysosomal membranes. [provided by RefSeq Jul 2008]

Gene Symbol:   LAPTM4A
Gene Name:   lysosomal protein transmembrane 4 alpha
Aliases:   HUMORF13, KIAA0108, LAPTM4, MBNT, Mtrp
RefSeq:   NC_000002.11 NT_015926.15
Ensembl:   ENSG00000068697
Entrez:   9741
Chromosome Mapping:   2p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.260000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download