PrimePCR™ SYBR® Green Assay: SLC25A19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028031

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011463
Assay Design:   Intron-spanning
Chromosome Location:   17:73273519-73274355question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000416858 ENST00000442286 ENST00000320362 ENST00000402418 ENST00000375261

Gene Information

This gene encodes a mitochondrial protein that is a member of the solute carrier family. Although this protein was initially thought to be the mitochondrial deoxynucleotide carrier involved in the uptake of deoxynucleotides into the matrix of the mitochondria further studies have demonstrated that this protein instead functions as the mitochondrial thiamine pyrophosphate carrier which transports thiamine pyrophosphates into mitochondria. Mutations in this gene cause microcephaly Amish type a metabolic disease that results in severe congenital microcephaly severe 2-ketoglutaric aciduria and death within the first year. Multiple alternatively spliced variants encoding the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC25A19
Gene Name:   solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19
Aliases:   DNC, MCPHA, MUP1, TPC
RefSeq:   NC_000017.10 NT_010783.15 NG_008274.1
Ensembl:   ENSG00000125454
Entrez:   60386
UniGene:   Hs.514470
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.500000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download