PrimePCR™ SYBR® Green Assay: TNNC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027999

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TNNC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TNNC2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011402
Assay Design:   Intron-spanning
Chromosome Location:   20:44451937-44452708question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000372557 ENST00000372555

Gene Information

Troponin (Tn) a key protein complex in the regulation of striated muscle contraction is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase the Tn-T subunit binds tropomyosin and Tn-C while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq Jul 2008]

Gene Symbol:   TNNC2
Gene Name:   troponin C type 2 (fast)
Aliases:   Not Available
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101470
Entrez:   7125
Chromosome Mapping:   20q12-q13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.480000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download