PrimePCR™ SYBR® Green Assay: GSG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027915

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011211
Assay Design:   Intron-spanning
Chromosome Location:   12:13238119-13240188question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000337630 ENST00000396302 ENST00000457134 ENST00000432710 ENST00000537302 ENST00000351606 ENST00000324458 ENST00000396310 ENST00000405543

Gene Information

Description Not Available

Gene Symbol:   GSG1
Gene Name:   germ cell associated 1
Aliases:   MGC111023, MGC3146
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000111305
Entrez:   83445
UniGene:   Hs.240053
Chromosome Mapping:   12p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.900000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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