PrimePCR™ SYBR® Green Assay: MED8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027819

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011007
Assay Design:   Intron-spanning
Chromosome Location:   1:43850717-43851744question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000290663 ENST00000372457 ENST00000372455

Gene Information

This gene encodes a protein that is one of more than 20 subunits of the mediator complex first identified in S. cerevisiae that is required for activation of transcription. The product of this gene also interacts with elongins B and C and CUL2 and RBX1 to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   MED8
Gene Name:   mediator complex subunit 8
Aliases:   ARC32, MGC17544, MGC19641
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000159479
Entrez:   112950
Chromosome Mapping:   1p34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.560000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download