This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027819
PrimePCR™ PreAmp for SYBR® Green Assay: MED8, Human
PrimePCR™ Template for SYBR® Green Assay: MED8, Human
This gene encodes a protein that is one of more than 20 subunits of the mediator complex first identified in S. cerevisiae that is required for activation of transcription. The product of this gene also interacts with elongins B and C and CUL2 and RBX1 to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.