PrimePCR™ SYBR® Green Assay: RAB3GAP2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010922
Assay Design:   Intron-spanning
Chromosome Location:   1:220375656-220379337question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000484658 ENST00000358951

Gene Information

The protein encoded by this gene belongs to the RAB3 protein family members of which are involved in regulated exocytosis of neurotransmitters and hormones. This protein forms the Rab3 GTPase-activating complex with RAB3GAP1 where it constitutes the regulatory subunit whereas the latter functions as the catalytic subunit. This gene has the highest level of expression in the brain consistent with it having a key role in neurodevelopment. Mutations in this gene are associated with Martsolf syndrome.[provided by RefSeq Oct 2009]

Gene Symbol:   RAB3GAP2
Gene Name:   RAB3 GTPase activating protein subunit 2 (non-catalytic)
Aliases:   DKFZp434D245, FLJ14579, KIAA0839, RAB3-GAP150, RAB3GAP150, p150
RefSeq:   NC_000001.10 NG_015837.1 NT_167186.1
Ensembl:   ENSG00000118873
Entrez:   25782
UniGene:   Hs.654849
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.590000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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