This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SLC35B4, Human
PrimePCR™ Template for SYBR® Green Assay: SLC35B4, Human
Glycosyltransferases such as SLC35B4 transport nucleotide sugars from the cytoplasm where they are synthesized to the Golgi apparatus where they are utilized in the synthesis of glycoproteins glycolipids and proteoglycans (Ashikov et al. 2005 [PubMed 15911612]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.