PrimePCR™ SYBR® Green Assay: KDM2B, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027770

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010881
Assay Design:   Intron-spanning
Chromosome Location:   12:121970812-121986784question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000377069 ENST00000377071 ENST00000446152 ENST00000538379 ENST00000545022 ENST00000541318 ENST00000543852 ENST00000541511 ENST00000539394 ENST00000539371 ENST00000397480 ENST00000536437 ENST00000397478 ENST00000261824

Gene Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box) which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains Fbls containing leucine-rich repeats and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene but the full-length nature of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   KDM2B
Gene Name:   lysine (K)-specific demethylase 2B
Aliases:   CXXC2, FBXL10, Fbl10, JHDM1B, PCCX2
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000089094
Entrez:   84678
UniGene:   Hs.524800
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.080000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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