PrimePCR™ SYBR® Green Assay: MAP3K6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027755

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAP3K6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAP3K6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010855
Assay Design:   Intron-spanning
Chromosome Location:   1:27685247-27685959question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000472410 ENST00000493901 ENST00000357582 ENST00000545447 ENST00000374040

Gene Information

This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7 but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene but its biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   MAP3K6
Gene Name:   mitogen-activated protein kinase kinase kinase 6
Aliases:   ASK2, MAPKKK6, MEKK6, MGC125653, MGC20114
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000142733
Entrez:   9064
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download