PrimePCR™ SYBR® Green Assay: SYBU, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010842
Assay Design:   Intron-spanning
Chromosome Location:   8:110590198-110592082question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000533895 ENST00000424158 ENST00000532779 ENST00000399066 ENST00000446070 ENST00000528331 ENST00000529175 ENST00000276646 ENST00000528647 ENST00000533065 ENST00000529690 ENST00000533171 ENST00000533394 ENST00000422135 ENST00000419099 ENST00000433638 ENST00000408908 ENST00000440310 ENST00000408889

Gene Information

Syntabulin/GOLSYN is part of a kinesin motor-adaptor complex that is critical for the anterograde axonal transport of active zone components and contributes to activity-dependent presynaptic assembly during neuronal development (Cai et al. 2007 [PubMed 17611281]).[supplied by OMIM Mar 2008]

Gene Symbol:   SYBU
Gene Name:   syntabulin (syntaxin-interacting)
Aliases:   FLJ20366, GOLSYN, KIAA1472, OCSYN, SNPHL
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000147642
Entrez:   55638
UniGene:   Hs.390738
Chromosome Mapping:   8q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 34.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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