This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027751
PrimePCR™ PreAmp for SYBR® Green Assay: TTC7A, Human
PrimePCR™ Template for SYBR® Green Assay: TTC7A, Human
The tetratricopeptide repeat (TPR) domain is defined by a degenerate consensus sequence of 34 amino acids. TPR domain-containing proteins such as TTC7A have diverse functions in cell cycle control protein transport phosphate turnover and protein trafficking or secretion and they can act as chaperones or scaffolding proteins (White et al. 2005 [PubMed 15718100]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.