PrimePCR™ SYBR® Green Assay: NFASC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027744

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NFASC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NFASC, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010820
Assay Design:   Intron-spanning
Chromosome Location:   1:204921179-204923361question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000446412 ENST00000403080 ENST00000404076 ENST00000401399 ENST00000505079 ENST00000404907 ENST00000513543 ENST00000430393 ENST00000367173 ENST00000367172 ENST00000367171 ENST00000367170 ENST00000338515 ENST00000339876 ENST00000338586 ENST00000295776 ENST00000539706 ENST00000360049 ENST00000367169

Gene Information

This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth neurite fasciculation and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein gliomedin ankyrin 3 (ankyrin-G) and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing and the full-length nature of some variants has not been determined.[provided by RefSeq May 2009]

Gene Symbol:   NFASC
Gene Name:   neurofascin
Aliases:   DKFZp686P2250, FLJ46866, KIAA0756, NF, NRCAML
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163531
Entrez:   23114
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download