This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027731
PrimePCR™ PreAmp for SYBR® Green Assay: DDX50, Human
PrimePCR™ Template for SYBR® Green Assay: DDX50, Human
DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this DEAD box protein family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21 also called RH-II/GuA have similar genomic structures and are in tandem orientation on chromosome 10 suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2 3 and 4. Alternative splicing of this gene generates multiple transcript variants but the full length nature of all the other variants but one has not been defined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.