PrimePCR™ SYBR® Green Assay: DDX50, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027731

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010786
Assay Design:   Intron-spanning
Chromosome Location:   10:70673880-70679632question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000373585 ENST00000541832

Gene Information

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation nuclear and mitochondrial splicing and ribosome and spliceosome assembly. Based on their distribution patterns some members of this DEAD box protein family are believed to be involved in embryogenesis spermatogenesis and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21 also called RH-II/GuA have similar genomic structures and are in tandem orientation on chromosome 10 suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2 3 and 4. Alternative splicing of this gene generates multiple transcript variants but the full length nature of all the other variants but one has not been defined. [provided by RefSeq Jul 2008]

Gene Symbol:   DDX50
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Aliases:   GU2, GUB, MGC3199, RH-II/GuB
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107625
Entrez:   79009
UniGene:   Hs.522984
Chromosome Mapping:   10q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.010000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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