PrimePCR™ SYBR® Green Assay: FAM107B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010746
Assay Design:   Intron-spanning
Chromosome Location:   10:14563964-14572393question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000378470 ENST00000181796 ENST00000468747 ENST00000378467 ENST00000378465 ENST00000378458 ENST00000478076 ENST00000378462 ENST00000378461 ENST00000496330 ENST00000479731 ENST00000468492 ENST00000452706 ENST00000489100 ENST00000494865 ENST00000475786 ENST00000488576 ENST00000442012 ENST00000482277 ENST00000472095

Gene Information

Description Not Available

Gene Symbol:   FAM107B
Gene Name:   family with sequence similarity 107, member B
Aliases:   C10orf45, FLJ45505, MGC11034, MGC90261
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000065809
Entrez:   83641
UniGene:   Hs.446315
Chromosome Mapping:   10p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.700000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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