This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027701
PrimePCR™ PreAmp for SYBR® Green Assay: DMD, Human
PrimePCR™ Template for SYBR® Green Assay: DMD, Human
The dystrophin gene is the largest gene found in nature measuring 2.4 Mb. The gene was identified through a positional cloning approach targeted at the isolation of the gene responsible for Duchenne (DMD) and Becker (BMD) Muscular Dystrophies. DMD is a recessive fatal X-linked disorder occurring at a frequency of about 1 in 3500 new-born males. BMD is a milder allelic form. In general DMD patients carry mutations which cause premature translation termination (nonsense or frame shift mutations) while in BMD patients dystrophin is reduced either in molecular weight (derived from in-frame deletions) or in expression level. The dystrophin gene is highly complex containing at least eight independent tissue-specific promoters and two polyA-addition sites. Furthermore dystrophin RNA is differentially spliced producing a range of different transcripts encoding a large set of protein isoforms. Dystrophin (as encoded by the Dp427 transcripts) is a large rod-like cytoskeletal protein which is found at the inner surface of muscle fibers. Dystrophin is part of the dystrophin-glycoprotein complex (DGC) which bridges the inner cytoskeleton (F-actin) and the extra-cellular matrix. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.