PrimePCR™ SYBR® Green Assay: UCHL5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010701
Assay Design:   Intron-spanning
Chromosome Location:   1:192992076-192993012question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000420791 ENST00000367455 ENST00000367454 ENST00000449480 ENST00000367450 ENST00000367451 ENST00000367448 ENST00000367449 ENST00000443327 ENST00000416915 ENST00000421683 ENST00000391991 ENST00000367452 ENST00000530098

Gene Information

Description Not Available

Gene Symbol:   UCHL5
Gene Name:   ubiquitin carboxyl-terminal hydrolase L5
Aliases:   CGI-70, INO80R, UCH-L5, UCH37
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000116750
Entrez:   51377
UniGene:   Hs.145469
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.440000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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