This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027681
PrimePCR™ PreAmp for SYBR® Green Assay: INPP4B, Human
PrimePCR™ Template for SYBR® Green Assay: INPP4B, Human
INPP4B encodes the inositol polyphosphate 4-phosphatase type II one of the enzymes involved in phosphatidylinositol signaling pathways. This enzyme removes the phosphate group at position 4 of the inositol ring from inositol 34-bisphosphate. There is limited data to suggest that the human type II enzyme is subject to alternative splicing as has been established for the type I enzyme. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.