PrimePCR™ SYBR® Green Assay: NRG2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: NRG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NRG2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010600
Assay Design:   Intron-spanning
Chromosome Location:   5:139260501-139266964question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000289422 ENST00000361474 ENST00000340391 ENST00000289409 ENST00000358522 ENST00000378238 ENST00000541337 ENST00000446269 ENST00000545385 ENST00000394770 ENST00000544729

Gene Information

This gene encodes a novel member of the neuregulin family of growth and differentiation factors. Through interaction with the ERBB family of receptors this protein induces the growth and differentiation of epithelial neuronal glial and other types of cells. The gene consists of 12 exons and the genomic structure is similar to that of neuregulin 1 another member of the neuregulin family of ligands. The products of these genes mediate distinct biological processes by acting at different sites in tissues and eliciting different biological responses in cells. This gene is located close to the region for demyelinating Charcot-Marie-Tooth disease locus but is not responsible for this disease. Alternative transcript variants encoding distinct isoforms have been described. [provided by RefSeq May 2010]

Gene Symbol:   NRG2
Gene Name:   neuregulin 2
Aliases:   DON1, HRG2, NTAK
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000158458
Entrez:   9542
Chromosome Mapping:   5q23-q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.040000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download