PrimePCR™ SYBR® Green Assay: AP3B2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027629

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AP3B2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AP3B2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010547
Assay Design:   Intron-spanning
Chromosome Location:   15:83345280-83346078question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000261722 ENST00000535348 ENST00000535359

Gene Information

Adaptor protein-3 (AP3) is a heterotetrameric vesicle-coat protein complex. Some AP3 subunits are ubiquitously expressed whereas others are expressed exclusively in neurons. The neuron-specific AP3 complex which includes AP3B2 is thought to serve neuron-specific functions such as neurotransmitter release (Grabner et al. 2006 [PubMed 16788073]).[supplied by OMIM Mar 2008]

Gene Symbol:   AP3B2
Gene Name:   adaptor-related protein complex 3, beta 2 subunit
Aliases:   DKFZp686D17136, NAPTB
RefSeq:   NC_000015.9 NT_077661.3
Ensembl:   ENSG00000103723
Entrez:   8120
Chromosome Mapping:   15q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.320000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download