PrimePCR™ SYBR® Green Assay: SIGLEC10, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027627

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010540
Assay Design:   Intron-spanning
Chromosome Location:   19:51917019-51917764question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000432469 ENST00000442846 ENST00000356298 ENST00000339313 ENST00000439889 ENST00000436984 ENST00000525998 ENST00000441969 ENST00000353836

Gene Information

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs or ITIMs. SIGLECs are typically expressed on cells of the innate immune system with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM Jul 2002]

Gene Symbol:   SIGLEC10
Gene Name:   sialic acid binding Ig-like lectin 10
Aliases:   MGC126774, PRO940, SIGLEC-10, SLG2
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000142512
Entrez:   89790
UniGene:   Hs.284813
Chromosome Mapping:   19q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.510000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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