This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027627
PrimePCR™ PreAmp for SYBR® Green Assay: SIGLEC10, Human
PrimePCR™ Template for SYBR® Green Assay: SIGLEC10, Human
SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs or ITIMs. SIGLECs are typically expressed on cells of the innate immune system with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM Jul 2002]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.