PrimePCR™ SYBR® Green Assay: VPS45, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010473
Assay Design:   Intron-spanning
Chromosome Location:   1:150054052-150054916question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000369130 ENST00000419023 ENST00000543996 ENST00000535106 ENST00000369128

Gene Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family and shows a high degree of sequence similarity to mouse rat and yeast Vps45. The exact function of this gene is not known but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins including inflammatory mediators. [provided by RefSeq Jul 2008]

Gene Symbol:   VPS45
Gene Name:   vacuolar protein sorting 45 homolog (S. cerevisiae)
Aliases:   H1, H1VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000136631
Entrez:   11311
UniGene:   Hs.443750
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.770000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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