PrimePCR™ SYBR® Green Assay: THOC5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027581

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010424
Assay Design:   Intron-spanning
Chromosome Location:   22:29939481-29940504question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000490103 ENST00000397873 ENST00000440771 ENST00000455450 ENST00000428374 ENST00000418021 ENST00000397872 ENST00000397871

Gene Information

Description Not Available

Gene Symbol:   THOC5
Gene Name:   THO complex 5
Aliases:   C22orf19, Fmip, PK1.3, fSAP79
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100296
Entrez:   8563
UniGene:   Hs.75361
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.610000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download