PrimePCR™ SYBR® Green Assay: CTNNB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010363
Assay Design:   Intron-spanning
Chromosome Location:   3:41267278-41268734question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000405570 ENST00000396183 ENST00000349496 ENST00000453024 ENST00000396185

Gene Information

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally this protein binds to the product of the APC gene which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC) pilomatrixoma (PTR) medulloblastoma (MDB) and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   CTNNB1
Gene Name:   catenin (cadherin-associated protein), beta 1, 88kDa
Aliases:   CTNNB, DKFZp686D02253, FLJ25606, FLJ37923
RefSeq:   NC_000003.11 NT_022517.18 NG_013302.1
Ensembl:   ENSG00000168036
Entrez:   1499
UniGene:   Hs.476018
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.180000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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