PrimePCR™ SYBR® Green Assay: CLK2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027552

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CLK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CLK2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010358
Assay Design:   Intron-spanning
Chromosome Location:   1:155236620-155237862question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000361168 ENST00000368361 ENST00000355560 ENST00000536801 ENST00000424156

Gene Information

This gene encodes a member of the CLK family of dual specificity protein kinases. CLK family members have been shown to interact with and phosphorylate serine- and arginine-rich (SR) proteins of the spliceosomal complex which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. Note that this gene is distinct from TELO2 gene (GeneID:9894) which shares CLK2 and hCLK2 symbol aliases in common with this gene but encodes a protein that is involved in telomere length regulation. [provided by RefSeq Jul 2008]

Gene Symbol:   CLK2
Gene Name:   CDC-like kinase 2
Aliases:   MGC61500, hCLK2
RefSeq:   NC_000001.10 NT_004487.19 NW_003315906.1
Ensembl:   ENSG00000176444
Entrez:   1196
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download