PrimePCR™ SYBR® Green Assay: LARP1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027537

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LARP1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LARP1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010317
Assay Design:   Intron-spanning
Chromosome Location:   4:128996104-128999056question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000326639 ENST00000512292 ENST00000508819 ENST00000394288 ENST00000432347 ENST00000264584 ENST00000441387 ENST00000427266 ENST00000354456

Gene Information

This gene encodes a protein containing domains found in the La related protein of Drosophila melanogaster. La motif-containing proteins are thought to be RNA-binding proteins where the La motif and adjacent amino acids fold into an RNA recognition motif. The La motif is also found in proteins unrelated to the La protein. Alternative splicing has been observed at this locus and three variants encoding distinct isoforms are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   LARP1B
Gene Name:   La ribonucleoprotein domain family, member 1B
Aliases:   DKFZp686L13217, LARP2, MGC117277, MGC75174
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138709
Entrez:   55132
Chromosome Mapping:   4q28.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.160000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download