PrimePCR™ SYBR® Green Assay: NOSTRIN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NOSTRIN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NOSTRIN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010228
Assay Design:   Intron-spanning
Chromosome Location:   2:169707789-169711910question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000458381 ENST00000317647 ENST00000445023 ENST00000397206 ENST00000397209 ENST00000421711 ENST00000444448

Gene Information

Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission inflammatory response and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production endothelial NO synthase (ENOS; MIM 163729) and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures thereby attenuating ENOS-dependent NO production.[supplied by OMIM Apr 2004]

Gene Symbol:   NOSTRIN
Gene Name:   nitric oxide synthase trafficker
Aliases:   DaIP2, MGC20702
RefSeq:   NC_000002.11 NT_005403.17 NW_003315909.1
Ensembl:   ENSG00000163072
Entrez:   115677
Chromosome Mapping:   2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.820000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download