PrimePCR™ SYBR® Green Assay: MYO7A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027477

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MYO7A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MYO7A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010173
Assay Design:   Intron-spanning
Chromosome Location:   11:76858937-76867050question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000409709 ENST00000409893 ENST00000458637 ENST00000409619 ENST00000358342 ENST00000343356 ENST00000341717 ENST00000343419 ENST00000545136

Gene Information

This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain an actin-binding domain a neck domain that interacts with other proteins and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness reduced vestibular function and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jul 2008]

Gene Symbol:   MYO7A
Gene Name:   myosin VIIA
Aliases:   DFNA11, DFNB2, MYOVIIA, MYU7A, NSRD2, USH1B
RefSeq:   NC_000011.9 NG_009086.1 NT_167190.1
Ensembl:   ENSG00000137474
Entrez:   4647
Chromosome Mapping:   11q13.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download