This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027477
PrimePCR™ PreAmp for SYBR® Green Assay: MYO7A, Human
PrimePCR™ Template for SYBR® Green Assay: MYO7A, Human
This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain an actin-binding domain a neck domain that interacts with other proteins and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness reduced vestibular function and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.