PrimePCR™ SYBR® Green Assay: PXK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027467

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PXK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PXK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010145
Assay Design:   Intron-spanning
Chromosome Location:   3:58368344-58376361question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000356151 ENST00000463280 ENST00000383715 ENST00000484288 ENST00000479241 ENST00000302779 ENST00000383716

Gene Information

PXK binds to the NaK-ATPase beta-1 (ATP1B1; MIM 182330) and beta-3 (ATP1B3; MIM 601867) subunits and modulates both NaK-ATPase enzymatic and ion pump activities (Mao et al. 2005 [PubMed 16135750]).[supplied by OMIM Mar 2008]

Gene Symbol:   PXK
Gene Name:   PX domain containing serine/threonine kinase
Aliases:   FLJ20335, MONaKA
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000168297
Entrez:   54899
Chromosome Mapping:   3p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.340000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download