PrimePCR™ SYBR® Green Assay: SLC30A6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC30A6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC30A6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010138
Assay Design:   Intron-spanning
Chromosome Location:   2:32431955-32445327question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000379343 ENST00000282587 ENST00000406369 ENST00000435660 ENST00000538303 ENST00000357055

Gene Information

Zinc functions as a cofactor for numerous enzymes nuclear factors and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family such as SLC30A6 permit cellular efflux of zinc (Seve et al. 2004 [PubMed 15154973]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC30A6
Gene Name:   solute carrier family 30 (zinc transporter), member 6
Aliases:   FLJ31101, MGC45055, MST103, MSTP103, ZNT6
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000152683
Entrez:   55676
Chromosome Mapping:   2p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.700000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download