PrimePCR™ SYBR® Green Assay: CCDC77, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027452

List Price:    $138.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010102
Assay Design:   Intron-spanning
Chromosome Location:   12:541090-542475question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000540180 ENST00000543504 ENST00000239830 ENST00000412006 ENST00000422000

Gene Information

Description Not Available

Gene Symbol:   CCDC77
Gene Name:   coiled-coil domain containing 77
Aliases:   MGC13183
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000120647
Entrez:   84318
UniGene:   Hs.631656
Chromosome Mapping:   12p13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 34.900000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download