This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: PTPN21, Human
PrimePCR™ Template for SYBR® Green Assay: PTPN21, Human
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP contains an N-terminal domain similar to cytoskeletal- associated proteins including band 4.1 ezrin merlin and radixin. This PTP was shown to specially interact with BMX/ETK a member of Tec tyrosine kinase family characterized by a multimodular structures including PH SH3 and SH2 domains. The interaction of this PTP with BMX kinase was found to increase the activation of STAT3 but not STAT2 kinase. Studies of the similar gene in mice suggested the possible roles of this PTP in liver regeneration and spermatogenesis. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.