This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027302
PrimePCR™ PreAmp for SYBR® Green Assay: LYPD6, Human
PrimePCR™ Template for SYBR® Green Assay: LYPD6, Human
Members of the LY6 protein family (see SLURP1; MIM 606119) such as LYPD6 have at least one 80-amino acid LU domain that contains 10 conserved cysteines with a defined disulfide-bonding pattern (Zhang et al. 2010 [PubMed 19653121]).[supplied by OMIM Apr 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.