This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NXF5, Human
PrimePCR™ Template for SYBR® Green Assay: NXF5, Human
This gene is one member of a family of nuclear RNA export factor genes. The encoded protein can bind RNA and is implicated in mRNA nuclear export. However this protein has lost several C-terminal protein domains found in other family members that are required for export activity and may be an evolving pseudogene. Alternatively spliced transcript variants have been described but most are candidates for nonsense-mediated decay (NMD) and may not express proteins in vivo. [provided by RefSeq Jul 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.