PrimePCR™ SYBR® Green Assay: MPP4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009652
Assay Design:   Intron-spanning
Chromosome Location:   2:202510104-202512525question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000409474 ENST00000315506 ENST00000396886 ENST00000428900 ENST00000409143 ENST00000447335 ENST00000359962 ENST00000315549 ENST00000374605

Gene Information

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) protein family with an N-terminal PDZ domain a central src homology 3 region (SH3) and a C-terminal guanylate kinase-like (GUK) domain. The protein is localized to the outer limiting membrane in the retina and is thought to function in photoreceptor polarity and the organization of specialized intercellular junctions. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   MPP4
Gene Name:   membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Aliases:   ALS2CR5, DLG6
RefSeq:   NC_000002.11 NG_008775.1 NG_012654.1 NT_005403.17
Ensembl:   ENSG00000082126
Entrez:   58538
Chromosome Mapping:   2q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download