This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027219
PrimePCR™ PreAmp for SYBR® Green Assay: MTMR9, Human
PrimePCR™ Template for SYBR® Green Assay: MTMR9, Human
This gene encodes a myotubularin-related protein that is atypical to most other members of the myotubularin-related protein family because it has no dual-specificity phosphatase domain. The encoded protein contains a double-helical motif similar to the SET interaction domain which is thought to have a role in the control of cell proliferation. In mouse a protein similar to the encoded protein binds with MTMR7 and together they dephosphorylate phosphatidylinositol 3-phosphate and inositol 13-bisphosphate. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.