PrimePCR™ SYBR® Green Assay: MTMR9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027219

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009496
Assay Design:   Intron-spanning
Chromosome Location:   8:11163850-11167124question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000221086 ENST00000526292

Gene Information

This gene encodes a myotubularin-related protein that is atypical to most other members of the myotubularin-related protein family because it has no dual-specificity phosphatase domain. The encoded protein contains a double-helical motif similar to the SET interaction domain which is thought to have a role in the control of cell proliferation. In mouse a protein similar to the encoded protein binds with MTMR7 and together they dephosphorylate phosphatidylinositol 3-phosphate and inositol 13-bisphosphate. [provided by RefSeq Jul 2008]

Gene Symbol:   MTMR9
Gene Name:   myotubularin related protein 9
Aliases:   C8orf9, DKFZp434K171, LIP-STYX, MGC126672, MTMR8
RefSeq:   NC_000008.10 NT_077531.4
Ensembl:   ENSG00000104643
Entrez:   66036
Chromosome Mapping:   8p23-p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.270000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download