This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027213
PrimePCR™ PreAmp for SYBR® Green Assay: RFX4, Human
PrimePCR™ Template for SYBR® Green Assay: RFX4, Human
This gene is a member of the regulatory factor X gene family which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1 X2 X3 and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3 but it does not interact with regulatory factor X1. Alternative splicing results in multiple transcript variants. [provided by RefSeq May 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.