This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027202
PrimePCR™ PreAmp for SYBR® Green Assay: FZD6, Human
PrimePCR™ Template for SYBR® Green Assay: FZD6, Human
This gene represents a member of the 'frizzled' gene family which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide a cysteine-rich domain in the N-terminal extracellular region and seven transmembrane domains but unlike other family members this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade thereby inhibiting the processes that trigger oncogenic transformation cell proliferation and inhibition of apoptosis. Alternative splicing results in multiple transcript variants some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.