This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027187
PrimePCR™ PreAmp for SYBR® Green Assay: FBXW12, Human
PrimePCR™ Template for SYBR® Green Assay: FBXW12, Human
Members of the F-box protein family such as FBXW12 are characterized by an approximately 40-amino acid F-box motif. SCF complexes formed by SKP1 (MIM 601434) cullin (see CUL1; MIM 603034) and F-box proteins act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box and they interact with ubiquitination targets through other protein interaction domains (Jin et al. 2004 [PubMed 15520277]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.