This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027142
PrimePCR™ PreAmp for SYBR® Green Assay: HERC6, Human
PrimePCR™ Template for SYBR® Green Assay: HERC6, Human
HERC6 belongs to the HERC family of ubiquitin ligases all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al. 2005 [PubMed 15676274]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.