PrimePCR™ SYBR® Green Assay: PDCD10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: PDCD10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PDCD10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009192
Assay Design:   Intron-spanning
Chromosome Location:   3:167405422-167413446question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000392750 ENST00000473645 ENST00000497056 ENST00000492396 ENST00000461494 ENST00000470131 ENST00000475915 ENST00000487947 ENST00000471885

Gene Information

This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25 and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   PDCD10
Gene Name:   programmed cell death 10
Aliases:   CCM3, MGC1212, MGC24477, TFAR15
RefSeq:   NC_000003.11 NG_008158.1 NG_008217.1 NT_005612.16
Ensembl:   ENSG00000114209
Entrez:   11235
Chromosome Mapping:   3q26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.790000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download