PrimePCR™ SYBR® Green Assay: SERPINI2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: SERPINI2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SERPINI2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009187
Assay Design:   Intron-spanning
Chromosome Location:   3:167170728-167183055question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000476257 ENST00000461846 ENST00000471111 ENST00000264677

Gene Information

The protein encoded by this gene is a member of the serine protease inhibitor (serpin) superfamily made up of proteins which play central roles in the regulation of a wide variety of physiological processes including coagulation fibrinolysis development malignancy and inflammation. The gene product may have a role in a growth-control possibly growth-suppressing pathway and when impaired may be involved in pancreatic carcinogenesis. The protein is a member of the plasminogen activator inhibitor-1 family a subset of the serpin superfamily whose members act as tissue-specific tPA inhibitors. Two alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SERPINI2
Gene Name:   serpin peptidase inhibitor, clade I (pancpin), member 2
Aliases:   MEPI, PANCPIN, PI14, TSA2004
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114204
Entrez:   5276
Chromosome Mapping:   3q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download